Fig. 7. Allicin treatment resulted in synergistic cytotoxicity with cisplatin. (A) MTT Assay- Cytotoxic effect of 2μg/ml cisplatin, 10μg/ml allicin and combination of 2μg/ml cisplatin and 10μg/ml (LD) allicin in absence and presence of 5mM NAC on A549 cells cultured in normoxia and 1% hypoxia for 72hrs. The bars represent the mean ± standard deviation calculated from three independent experimental values. * represents p< 0.05 and ** represents p< 0.005. (B) Western blot analysis of total PARP, cleaved PARP and LC3 in A549 cells treated with 2μg/ml cisplatin (Cis), 10μg/ml allicin (LD) and combination of 2μg/ml cisplatin and 10μg/ml allicin (Cis + LD) in absence and presence of antioxidant NAC (5mM) and cultured in normoxia and 1% hypoxia for 48hr. β-actin is used as loading control. UT= untreated.